Kugelmeiers cell culture plates – Sphericalplate 5D (SP5D)

Functional principle

Standardized, uniform, size controlled spheroids in high yield.

750 cell clusters with a single pipette move - 9,000 per plate.

When developing the SP5D, the goal was to create the most realistic physiological environment possible for growing standardized spheroids with the possibility of cell-to-cell communication without external interfering signals. At the same time, the plate should allow real-time imaging and monitoring within the platform, be scalable and convenient to use.

These requirements are met by the following features of the SP5D:

Patented, three-dimensional geometry with rounded bottoms of the microwells, resulting in scaffold-free, regular spheroid formation while keeping the aggregate in the center no sharp edges at the bottom of the plate to reduce surface-induced, non-physiological differentiation sharp edges at the top of the microwells to leave no cell behind special angle of the microwells to allow cells descending in consistent numbers without an additional centrifugation step patented surface coating to prevent cell-surface signaling during development and eliminates the need for plate pre-treatment Surface coating featuring low protein-adhesion to allows perfect cell aggregation Aggregate size control through the geometric dimensions of the microwells to avoid hypoxia Standardization of geometric cluster size for clinical applications Selection of COC (cyclic olefin copolymers) material to enable real-time imaging with less background noise

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